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大肠肿瘤中p53和bcl-2蛋白的表达
研究p53和bcl-2在大肠肿瘤发生中的作用。方法：应用免疫组化S-P法分别检测大肠正常粘膜、腺瘤及腺癌中p53和 bcl-2表达。结果：大肠腺癌p53表达率高于腺瘤组（P＜0.05），p53表达与大肠癌的临床病理因素无关（P＞0.05），bcl-2在正常粘膜基底部上皮细胞表达，在腺瘤（77.5%）和腺癌中（55%）bcl-2表达差异显著(P＜0.01)，高分化腺癌bcl-2表达率(73.7%)高于差分化腺癌(41.2%)，在腺瘤和腺癌中bcl-2和p53呈负相关。结论：p53基因改变在大肠腺瘤癌变中起重要作用，大肠癌的发生与bcl-2基因在大肠腺瘤和腺癌中细胞凋亡抑制有关。
中图分类号 R735.34 文献标识码 A
文章编号 1001-7399(2000)03-0214-03
p53 and bcl-2 oncoprotein expression in colorectal tumor
Cao Liyu Zhang Hongfu Gong Xiyu Meng Gang
（Dept of Pathol，Anhui Medical University，Hefei 230032）
ABSTRACT Purpose To study the role of p53、bcl-2 in colorectal tumorigenesis.Methods Using immunohistochemistry S-P method，the expression of p53 and bcl-2 were examined in normal colonic mucosas，hyperplastic polyps，adenomas and adenocarcinomas.Results The expression rate of colorectal adenocarcinomas was significant higher than that of adenomas (P＜0.05).No significant correlation was observed between p53 expression and clinicopathological manifestations (P＞0.05). bcl-2 was expressed in the basal epithelial cells of the lower colonic cypts in mucosa and hyperplastic polyps.Significant difference of bcl-2 expression was found between adenomas and adenocarcinomas(P＜0.05).bcl-2 expression of well differentiated carcinomas was significant higher than that of poorly differentiated carcinomas (P＜0.01).An inverse correlation was found between bcl-2 and p53 expression in adenomas and in adenocarcinomas (P＜0.01).Conclusion Alteration of p53 gene is involved mainly in the transformation from benign adenoma to adenocarcinoma, colorectal carcinogenesis is related to the inhibition of apoptosis.
KEY WORDS colonic neoplasms; rectal neoplasms；protein p53; protein,bcl-2;apoptosis; immunohistochemistry
