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大肠癌中CD44V6、p53、PCNA的表达及意义
探讨CD44V6、p53、PCNA在大肠癌中的表达与病理及生理学行为之间的关系。方法 采用免疫组化S-P法检测CD44V6、p53、PCNA在51例大肠癌中的表达。结果 51例大肠癌CD44V6阳性表达率41.18%(21/51)。CD44V6在低分化大肠癌的检出率为53.85%(7/13)明显高于高分化大肠癌36.84%(7/19)(P＜0.05)，在浆膜外的阳性表达率53.38%(8/15)明显高于肌层33.33%(2/6)(P＜0.05)，有淋巴结转移者阳性表达率为63.16%(12/19)明显高于无淋巴结转移者31.25%(10/32)(P＜0.05)。p53在51例大肠癌中阳性检出率为50.59%(26/51)，其表达与大肠癌的组织类型、分化程度和淋巴结转移未见明显相关。而与浸润深度呈正相关，p53在浆膜外层的阳性表达率为53.33%(8/15)明显高于肌层33.33%(2/6)(P＜0.05)。PCNA的表达与病理分级、浸润深度和淋巴结转移未见显著相关性(P＞0.05)。结论 CD44V6的表达与大肠癌的临床病理生理学行为密切相关，而p53与大肠癌浸润深度关系密切。因此CD44V6和p53蛋白可为一个准确预测大肠癌预后的生物学指标。而PCNA作为大肠癌预后的标记物不够理想。
中图分类号：R735.34 文献标识码：A
文章编号：1000-7431(2000)05-0362-03
RELATIONSHIP BETWEEN EXPRESSION OF CD44V6,P53,PCNA AND THE PROGNOSIS OF COLORECTAL CARCINOMA
LIU Jing-xian, LI Ji-you,ZHANG Pei-rong, et al.
(Department of Pathology,School of Clinical Oncology, Beijing Medical University, Beijing, 100036,China)
Abstract：Objective To study the relation between the expression of CD44V6, p53 and PCNA and the prognosis of colorectal carcinoma. Methods Immnohistochemical method was used to detect the expression or CD44V6, p53 and PCNA in 51 patients with colorectal cacinoma.Results The positive rate of CD44V6 was 41.18%(21/51). It was 53.85%(7/13) in low differentiated colorectal carcinoma,markedly higher than that in highly differemtiated carcinoma (36.84%,7/19)(P＜0.05).The expression rate outside of serosa was 53.34%(8/15), markedly higher than that in muscular layer (33.33%,2/6)(P＜0.05)Also, it was much higher in carcinoma with lymph node metastasis (63.16%, 12/19) than that in carcinoma without lymph node metastasis (31.25 %,10/32)(P＜0.05).The positive rate of p53 was (50.59% 26/51) and was only related with the depth of infiltration of carcinoma. The expression of p53 in the outer layer of serosa was (53.33%, 8/15),apparently higher than that in muscular layer (33.33%,2/6)(P＜0.05),while the expression of PCNA was not related to pathological grade, depth of infiltration and lymph node metastasis (P＜0.05).Conclusion The expression of CD44V6 and p53 is correlated with progression and metastasis of colorectal carcinoma, so CD44V6 and p53 may be used as a hopeful marker for predicting the metastastic potential of colorectal carcinoma and the prognosis of these patients.
Key words：Colorectal neoplasms; Immunohistochemistry; Neoplasm metastasis; Gene products,p53; PCNA； CD44V6
大肠癌是我国死亡率较高的恶性肿瘤之一，大肠癌患者主要死亡原因是术后转移。临床上尚无可靠的方法预测预后，因此探讨大肠癌转移的分子机制，寻找与大肠癌转移相关的生物标记是大肠癌基因研究和临床研究的重要课题之一。作者选择了CD44V6、p53、PCNA对大肠癌进行标记，并对其表达情况及其临床应用的价值进行探讨。
