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应用半巢式PCR检测大肠癌组织及患者粪便中bcl-2基因的重排
研究大肠癌中bcl-2基因重排的规律，探讨通过粪便途径的基因诊断筛查大肠癌的可行性。方法 应用半巢式PCR技术检测28例大肠癌患者癌组织及粪便中bcl-2基因重排。结果 28例大肠癌组织标本中24例(85.7%)检出bcl-2重排，粪便中检出17例bcl-2重排，其癌组织标本中bcl-2均有重排。癌组织中bcl-2基因重排在大肠癌早期即已出现，随着病程演变，这种异常表现增多趋势。结论 bcl-2基因参与大肠癌发生发展的调节，并在大肠癌细胞的增殖、进展及转移中起一定作用。存在基因异常的癌细胞的绝大多数能够通过粪便标本得以检出。bcl-2半巢式PCR灵敏、特异，具有实际应用价值。
The detection of the rearrangements of bcl-2 gene in the cancer tissues and the stool of the patients with colorectal carcinoma by semi-nest PCR
LUO Chengyu, LI Shiyong, ZHU Xueguang.
General Hospital of Beijing Military Command, Beijing 100700
【Abstract】Objective To study the regularity of the rearrangement of bcl-2 in colorectal carcinoma and probe into the feasibility of gene diagnosis screening for colorectal carcinoma by way of stool.Method The rearrangements of bcl-2 gene in the cancer tissues and the stool of 28 patients with colorectal carcinoma were analyzed by semi-nest PCR.Results The positive rate of the rearrangements of bcl-2 in cancer tissues and in the stool was 85.7% and 70.8% respectively. The rearrangement of bcl-2 had emerged in the early stage of colorectal carcinoma. With the development of the course of disease, the rearrangement of bcl-2 showed an increased tendency.Conclusions bcl-2 gene involves in the regulation of the development of colorectal carcinoma and plays a role in the proliferation, progression and metastasis of the colorectal carcinoma. Most of the cancer cells with the abnormality of gene are detectable by way of stool. The semi-nest PCR for bcl-2 is sensitive and specific, and is of a practical value.
【Key words】 Colorectal neoplasms Stool DNA Bcl-2 gene Semi-nest PCR
我们应用高敏、特异的半巢式PCR技术对大肠癌组织及患者粪便中bcl-2基因的重排进行检测，旨在研究大肠癌中bcl-2基因重排的规律，并探讨通过粪便途径的基因诊断筛查大肠癌的可行性。
对象与方法
1、一般资料 经手术病理证实的大肠癌28例，其中男13例，女15例，年龄34～74岁。手术切除后立即取小块组织液氮速冻。粪便标本在术前留取、存于-70℃。
