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大肠癌组织中类固醇激素受体与淋巴结转移及预后的相关性
研究大肠癌组织中类固醇激素受体与淋巴结转移及预后的相 关性。方法 应用免疫组化ABC法检测99例大肠癌组织中的类固醇激素受体 。结果 大肠癌组织中有类固醇激素受体表达，其中ER、PgR、AR阳性率分 别为32.2%、38.4%、30.3%，类固醇激素受体阳性率与组织学分化程度成正相关，且类固 醇激素受体阳性患者淋巴结转移率低，预后好。结论 类固醇激素受体在反 映淋巴结转移率及预后方面与组织学分化程度有一致性。
The relativity of steroid hormone receptor in tissue of large i ntestine carcinoma with lymphatic metastasis and prognosis
Liu Haiyi,Li ang Xiaobo,Xue Yangqun,et al
（Shanxi Tumor Hospital,Taiyuan，0300 13,China）
Abstract：Objective To explore the relativity of steroid hormone receptor (SHR) in the tissue of large intestinal carcinoma with lymphati c metastasis and prognosis.Method 99 cases of carcinoma of large i ntestine were examined by the immunohistochemical ABC method.Results The positive rates of ER,PgR and AR were 32.3%,38.3% and 30.3% respectively,an d there was a direct correlation between the histological degree of differentiat ion and positive rate of SHR.Those whose SHR was positive had a lower rate of ly mphnode metastasis and a better prognosis.Conclusion The result sho wed that expression of SHR in the tissue of large intestinal carcinoma reflects the lymphatic metastasis and prognosis just in concordance with that of histoche mically differentiating degree.
Key words：Carcinoma of large intestine; Steroid hormone receptor; Lymph node metastasis; Prognosis
本文采用免疫组化ABC法对99例大肠癌石蜡切片进行回顾性研究，测定类固醇激素受体在大肠癌组织中的分布，重点研究其与淋巴结转移、预后的相关性及临床意义。
1 资料与方法
对我院1984年1月至1985年8月临床病理资料完整的99例直肠癌根治标本蜡块采用免疫组化AB C法进行回顾性ER、PgR、AR检测研究，其中男性56例、女性43例，所用ABC试剂盒为Vocter 公司生产。试剂为上海华美公司进口分装，每次染色均设立阳性与阴性对照。
ER、PgR、AR阳性标准：细胞质或核内有棕色颗粒为阳性，共分三级，切片内阳性细胞与肿 瘤细胞总数的比例≥20%(+)，≥40%()，≥60%()。
按全国大肠癌诊治规范进行组织学分类，根据肿瘤细胞分化程度分为三级：高分化腺癌(包 括乳头状腺癌)56例，中分化腺癌25例，低分化腺癌(包括粘液腺癌)18例。
