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bcl-2，c-myc和p53在大肠腺瘤和大肠癌中的表达
探讨癌基因bcl-2，c-myc和p53在大肠腺瘤与大 肠癌中的表达情况及与临床病理特征的关系。方法 采用免疫组化ABC法， 检测63例大肠癌和40例大肠腺瘤的bcl-2，c-myc和p53表达。结果 bcl -2，c-myc和p53在大肠腺瘤中的表达分别为72.7%(24/33)、62.2%(23/37)和45.0%(18/40) ，而在大肠癌中的表达则依次为34.0%(16/47)、82.1%(32/39)和84.1%(53/63)。三个癌基因 在大肠腺瘤和大肠癌中的表达均有显著的统计学意义(P＜0.01)，但三个癌基因的表达与 临床病理特征无显著的相关性。结论 bcl-2基因的异常激活在肿瘤的发 展和演变出现较早，而c-myc和p53的表达则较迟，且两者起协同作用。
分类号：R735.3+4 文献标识码：A
文章编号：1001-8174(2000)02-0081-02
Expression of bcl-2，c-myc and p53 in Colorectal Adenoma and Carcinoma
SUN Yan-xiang ZHOU Han-gao CHEN Chong-jia FENG Wei-ying
（Shibei Hospi tal of Shanghai,Shanghai 200435,China）
SHI Ying-qiang ZHENG Song-guo LUO Jian-min
（Cancer Hospital,Shanghai Medical Univers ity,Shanghai 200032,China）
Abstract：Objective To investigate the expressions of on cogenes bcl-2，c-myc and p53 in colorectal adenoma and adenocarcinoma,and the relationship between the expression of oncogene and clinical pathological features.Method Expression of oncogene bcl-2 were determinated in 33 cases of colorectal adenomas and 47 cases of colorectal carcinomas by the immunohistochemical method(ABC method).Expression of p53 in 40 cases of colotectal a denomas and 63 cases of colorectal carcinomas,in addition to c-myc protein in 3 7 cases of adenoma and 39 cases of colorectal adenocarcinomas were also determinated.Results The positive expression rates of bcl-2 in colorec tal adenoma and adenocarcinoma were 72.2%(24/33) and 24.2%(16/47) respectively,and those of p53 were 45.0%(18/40) and 84.1%(53/63) respectively.The Positive sta ining rates of c-myc in colorectal adenoma and adenocarcinoma were 62.7%(23/37) and 82.1%(30/39) respectively.There were significant differences between the expressions of these three oncogenes in colorectal adenoma and in adenocarcinoma( P＜0.01).However,the expression of these three oncogenes were not correlated with clinical pathological features.Conclusions The abnormal activation in bcl-2 takes place earlier during the development and progression of colorectal adenocarcinoma.But c-myc and p53 express later and cooperate with each other in progression of colorectal adenocarcinoma.
Key words：bcl-2;p53;c-myc;Cellar apoptosis;Colorectal carcino mas
业已证明，bcl-2抑制由野生型p53激发的细胞凋亡，且突变的p53也显示抑制细胞凋亡 。而c-myc与p53的共同表达在肿瘤的发展过程中可能起协同作用。大肠肿瘤提供了研究肿瘤的理想模式，即大多数癌似乎来源于腺瘤。并可分析各期肿瘤的发展情况。作者分析了bcl-2和c-myc在正常粘膜、腺瘤和癌肿时的表达情况。
